Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: The γ-carboxylated protein GAS6 is expressed by osteoblasts and activates its receptors on pre-osteoclasts. a Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. b Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclast cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6; n = 4). c Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3 h and treated with GAS6 (200 ng/mL) for the indicated times. Total AKT, MerTK, and AXL were used as loading controls. d Quantification of P-AKT (S473), P-MerTK (Y753), and P-AXL (Y702) normalized to the amount of total protein ( n = 3). Results represent the mean ± SEM

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: TAM signaling and γ-carboxylated GAS6 signaling promotes osteoclast formation in culture. Representative TRAP staining (left) and quantification of the TRAP + osteoclast area (right) in WT osteoblast (OB) and bone marrow cell (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 ( a ) or R428 ( b ), at the indicated concentrations. c–f Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant γ-carboxylated GAS6 at the indicated concentrations for up to 6 days. c Representative TRAP staining at day 5 and 6 of differentiation. d Quantification of the TRAP + osteoclast area at day 4, 5, and 6 of differentiation. e Quantification of the number of TRAP + multinucleated osteoclasts at day 4, 5, and 6 of differentiation. f Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s posttests was used in ( a , b ) and ( d – f ). *** P < 0.001, ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Staining, Derivative Assay, Cell Culture, Recombinant

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: Gamma-carboxylated GAS6 impacts on osteoclast differentiation and fusion. a–d Gene expression analysis by qPCR of osteoclast differentiation markers Acp5 (TRAP), Clcn7 , Ctsk , and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant γ-carboxylated GAS6 at the indicated concentrations for 2, 4, and 6 days ( n = 4 per condition). e Schematic representation of the assay used to assess the impact of γ-carboxylated GAS6 on pre-osteoclast fusion in culture using a conditionally activated tdTomato (Tom) reporter (created with BioRender). f Representative pictures of live osteoclast cultures at the indicated time and concentration of recombinant γ-carboxylated GAS6. The stars indicate the presence of fusion events (Tom + cells) in presence of GAS6 at Day 4. g Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation ( n = 16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s posttests was used in ( a – d ) and ( g ). *** P < 0.001, ** P < 0.01

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Gene Expression, Derivative Assay, Cell Culture, Recombinant, Concentration Assay

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: GAS6 promotes osteoclast formation and bone resorption in vivo. a–j Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. GAS6 concentration in the serum ( a ) and bone marrow cavity ( b ) ( n = 4). c Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. d Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections ( n = 15–17). e–h μCT analysis of the distal femur trabecular bone ( n = 12). e Representative μCT images. f Quantification of trabecular bone volume (BV/TV). g Quantification of trabecular bone surface density (BS/TV). h Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. i–j Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male ( n = 19–20). i Representative pictures of TRAP staining. j Number of osteoclasts per bone perimeter (N.Oc/B.Pm) and osteoclast surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in ( a , b , d , f – h and j ). *** P < 0.001, ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: In Vivo, Transgenic Assay, Concentration Assay, Staining, Derivative Assay

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: Increased bone mass in male mice lacking γ-carboxylation in osteoblasts. a Gene expression analysis by qPCR of Ggcx and Vkorc1 in bone marrow derived monocytes (BMMC), osteoclasts (OCL), proliferating pre-osteoblasts (pre-OB), and mineralized osteoblast (OB) cultures ( n = 3). b Protein expression in liver (Liv) and bone cells by Western blot. GGCX was analyzed on a 7.5% SDS Tris Glycine gel using 20 μg of extracts, while VKORC1 was resolved on a 10% SDS Tris Tricine gel using 10 μg of extracts. c–g Six-month-old Ggcx ff and Ggcx ff ;OCN-Cre male mice were analyzed ( n = 7–10). c Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. d Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections. e Representative μCT images of the distal femur trabecular bones. f Quantification of trabecular bone volume (BV/TV) and trabecular bone surface density (BS/TV) from the μCT data. g Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness respectively; Conn.Dn., connectivity density. Unpaired, 2-tailed Student’s t test was used in ( d ), ( f ), and ( g ). *** P < 0.001, ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Gene Expression, Derivative Assay, Expressing, Western Blot, Staining

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: Reduced osteoclast number and surface in Ggcx ff ;OCN-Cre male mice. a–h Bone histomorphometry analysis of lumbar vertebrae in six-month-old Ggcx ff and Ggcx ff ;OCN-Cre male mice ( n = 6–10). a Representative pictures of calcein double labeling and toluidine blue staining. b Mineral apposition rate (MAR). c Bone formation rate over bone surface (BFR/BS). d Number of osteoblasts per bone perimeter (N.Ob/B.Pm). e Osteoblast surface over bone surface (Ob.S/BS). f Representative pictures of TRAP staining. g Number of osteoclasts per bone perimeter (N.Oc/B.Pm). h Osteoclast surface over bone surface (Oc.S/BS). i Fasting serum CTx levels ( n = 12–17). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in ( b – e ) and ( g – i ). ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Labeling, Staining

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: Ggcx inactivation impairs the ability of osteoblasts to support osteoclastogenesis ex vivo. a Representative TRAP staining of osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of prostaglandin E 2 (PGE 2 ; 10 –6 mol/L) and 1,25 vitamin D 3 (VitD 3 ; 10 –8 mol/L). Ggcx ff osteoblasts were transduced with either Ad-GFP (control) or Ad-Cre (knockout) before the addition of the WT bone marrow cells. b Quantification of the number of TRAP + osteoclasts per well (Nb of OCL/well) ( n = 3). c Representative TRAP staining of osteoblasts and bone marrow cells co-cultures at day 8. Control ( Ocn + / + ) or osteocalcin-deficient ( Ocn -/- ) osteoblasts were cultured with WT bone marrow cells. d Quantification of the number of TRAP + osteoclasts per well (Nb of OCL/well) ( n = 3). e Gene expression analysis by qPCR in Ggcx ff + Ad-GFP and Ggcx ff + Ad-Cre osteoblasts cultured in presence (+) or absence (–) of PGE 2 and VitD 3 for 6 days. Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in ( b ) and ( d ). Two-way ANOVA with Bonferroni’s posttests was used in ( e ). *** P < 0.001, ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Ex Vivo, Staining, Transduction, Control, Knock-Out, Cell Culture, Gene Expression

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: The γ-carboxylated protein GAS6 is expressed by osteoblasts and activates its receptors on pre-osteoclasts. a Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. b Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclast cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6; n = 4). c Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3 h and treated with GAS6 (200 ng/mL) for the indicated times. Total AKT, MerTK, and AXL were used as loading controls. d Quantification of P-AKT (S473), P-MerTK (Y753), and P-AXL (Y702) normalized to the amount of total protein ( n = 3). Results represent the mean ± SEM

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

Journal: Bone Research

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice

doi: 10.1038/s41413-026-00528-2

Figure Lengend Snippet: TAM signaling and γ-carboxylated GAS6 signaling promotes osteoclast formation in culture. Representative TRAP staining (left) and quantification of the TRAP + osteoclast area (right) in WT osteoblast (OB) and bone marrow cell (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 ( a ) or R428 ( b ), at the indicated concentrations. c–f Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant γ-carboxylated GAS6 at the indicated concentrations for up to 6 days. c Representative TRAP staining at day 5 and 6 of differentiation. d Quantification of the TRAP + osteoclast area at day 4, 5, and 6 of differentiation. e Quantification of the number of TRAP + multinucleated osteoclasts at day 4, 5, and 6 of differentiation. f Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s posttests was used in ( a , b ) and ( d – f ). *** P < 0.001, ** P < 0.01, * P < 0.05, ns: non-significant

Article Snippet: GAS6 from serum and osteoblast supernatant were quantified using a mouse GAS6 ELISA (DuoSet ELISA DY986, R&D Systems, Minneapolis, MN) as we previously described.

Techniques: Staining, Derivative Assay, Cell Culture, Recombinant

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: AXL is highly expressed on ID8-derived cell lines. A. Stacked histogram plot showing expression of AXL on different passages of the ID8 cell lines compared to the FMO. B. Barplot showing the change in median fluorescence intensity of AXL (dMFI) in the different ID8 cell lines. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. C – H. Immunofluorescence images of the different ID8 cell lines at 40X magnification. Blue, DAPI; Red, AXL. I. Barplot showing the kinetic changes in the concentration of serum GAS6 (pg/mL) of P3 ID8 Thy1.1 tumor-bearing mice across weeks 1–6 post-inoculation and age-matched naïve mice. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. *, p < 0.05.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Derivative Assay, Expressing, Fluorescence, Immunofluorescence, Concentration Assay

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: High expression of AXL and GAS6 mRNA in OC patients correlates with worse outcome. A-D. Kaplan-Meier plots showing the hazard ratio (HR) and logrank P value in OC patients for A. AXL mRNA for overall survival (OS) ( AXL- high: n = 169, AXL- low: n = 204); B. GAS6 mRNA for OS ( GAS6- high: n = 125, GAS6- low: n = 248); C. GAS6 mRNA for recurrence-free survival (RFS) ( GAS6- high: n = 122, GAS6- low: n = 55); D. mean expression of AXL and GAS6 mRNA for OS ( AXL and GAS6 -high: n = 203, AXL and GAS6- low: n = 170). E. Heatmap showing high AXL or GAS6 mRNA expression in different tissues from OC patients. F. Heatmap showing high AXL or GAS6 mRNA expression in platinum-resistant or sensitive OC patients.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Expressing

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: AXL is highly expressed on ID8-derived cell lines. A. Stacked histogram plot showing expression of AXL on different passages of the ID8 cell lines compared to the FMO. B. Barplot showing the change in median fluorescence intensity of AXL (dMFI) in the different ID8 cell lines. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. C – H. Immunofluorescence images of the different ID8 cell lines at 40X magnification. Blue, DAPI; Red, AXL. I. Barplot showing the kinetic changes in the concentration of serum GAS6 (pg/mL) of P3 ID8 Thy1.1 tumor-bearing mice across weeks 1–6 post-inoculation and age-matched naïve mice. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. *, p < 0.05.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Derivative Assay, Expressing, Fluorescence, Immunofluorescence, Concentration Assay

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: High expression of AXL and GAS6 mRNA in OC patients correlates with worse outcome. A-D. Kaplan-Meier plots showing the hazard ratio (HR) and logrank P value in OC patients for A. AXL mRNA for overall survival (OS) ( AXL- high: n = 169, AXL- low: n = 204); B. GAS6 mRNA for OS ( GAS6- high: n = 125, GAS6- low: n = 248); C. GAS6 mRNA for recurrence-free survival (RFS) ( GAS6- high: n = 122, GAS6- low: n = 55); D. mean expression of AXL and GAS6 mRNA for OS ( AXL and GAS6 -high: n = 203, AXL and GAS6- low: n = 170). E. Heatmap showing high AXL or GAS6 mRNA expression in different tissues from OC patients. F. Heatmap showing high AXL or GAS6 mRNA expression in platinum-resistant or sensitive OC patients.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Expressing

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Staining, Derivative Assay, Cell Culture, Recombinant

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Gene Expression, Derivative Assay, Cell Culture, Recombinant, Concentration Assay

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Transgenic Assay, Concentration Assay, Staining, Derivative Assay

Journal: Cancer discovery

Article Title: AXL inhibition in macrophages stimulates host- versus -leukemia immunity and eradicates naive and treatment resistant leukemia

doi: 10.1158/2159-8290.CD-20-1378

Figure Lengend Snippet: (A) Prognostic value of GAS6 expression in acute myeloid leukemia (TCGA LAML, n=173). Data was generated using the KaplanScan mode from the R2 Genomics Analysis and Visualization Platform (http://r2.amc.nl). Survival analysis by log-rank (Mantel–Cox) test.

Article Snippet: Samples were then analyzed using the Mouse Gas6 DuoSet ELISA (DY986: R&D Systems), according to the manufacturer’s instructions.

Techniques: Expressing, Generated